Revista Cubana de Farmacia

Radiotratamiento de mafenida10 % Crema

The application of ioninzing radiations with decontaminant ends is an alternative to guarantee the microbiological quality of many pharmaceuticals. The radiotreatment with mafenide 10 % cream, which did not meet the microbiological quality specifications established in the country, was carried out. The microbial charge was determined and a dosage study of the product was conducted, evaluating 3, 5, 7, and 10 KGy of gamma radiations with a Co60 radioisotopic source. Microbiological and chemicophysical evaluations were carried out before and after the treatment. It was decided to irradiate the product with 3 KGy as a minimum dose, in a continual way and in the "Product I" plant of the Food Industry Research Institute.

Determinación de primidona por valoración potenciométrica en suspensión oral de primidona

Primidone oral suspension is used as an antiepileptic and anticonvulsive. The quantitative determination of primidone oral suspension may be carried out, according to the literature review, by the gaseous chromatography method and by the Kejldhal method. The results are reproducible and accurate in both; however, the first requires an expensive equipment, which is not always available in our small laboratories, and the second one is long and laborious. Therefore, it was developed a simpler and less laborious method able to quantify the amount os primidone in the suspension and to meet the industry's requirements. To this end, it was created a method of potentiometric residual assessment, and a basic hydrolisis with sodium hydroxide 0.5 mol/L was previously carried out. The factibility of the method proposed was checked and it proved to be specific, precise and exact. The analytical results were compared with those obtained by the methods reported in literature. A statistical study was conducted and the equivalence between both methods was demonstrated.

Clonazepan: Estudio preclínico de una formulación nacional

Se estudió comparativamente 2 formulaciones de clonazepán, la formulación nacional tabletas 1 mg lote 2003, en relación con el producto importado RivotrilR 0,5 mg de La Roche lote 818997, España. Para ello se evaluó la actividad anticonvulsivante al inducir convulsiones por pentilentetrazol; y la coordinación motora y el efecto miorrelajante, mediante el método de varilla rotatoria en ratones albinos suizos en ambos casos, procedentes de la colonia del laboratorio Control Biológico. Los 2 productos resultaron activos frente a las convulsiones, siendo el nacional más potente que el importado; para los otros 2 parámetros no aparece diferencia significativa. De las formulaciones estudiadas, con el producto nacional se alcanzó un margen terapéutico superior.

A comparative study of 2 clonazepam formulations, the national formulation in tablets 1 mg, batch 2 003, and the RivotrilR 0.5 mg imported from La Roche, batch 818 997, Spain, was carried out. Their anticonvulsant activity was evaluated by inducing convulsions with pentylenetetrazole; whereas the motor coordination and the myorelaxing effect were evaluated by using the rotatory rod method in Swiss albinic mice from the Biological Control Lab colony, in both cases. The two products were active to convulsions, but the national one was stronger than the imported one. No significant difference was observed in the other two parameters. Of the formulations studied a higher therapeutic margin was obtained with the national product.

Dosis letales de sulfato de quinina y de quinindina en el ratón: Influencia del sexo

The influence of sex on the quinine sulphate and quinidine lethality in mice was studied by two expiermental series: dose calculation according to Litchfield and Wilcoxon's methods, and observation of mortality produced by the administration of a unique prefixed dose of each drug. The results of the first series were processed as it is described in the above mentioned method (limits, lineality, parallelism, and relative potency). In the second series, it was used the Fisher's exact probability test. Similar lethal doses values were obtained with both drugs in connection with sex, but a significant differences was found between males and females. It was concluded that the female mouse was much more sensitive to the lethal effect of the drugs studied. Some factors that may influence on the effective doses values of these drugs are analyzed.

Determinación de vitamina (a -Tocoferol) en Pinus Caribaea Morelet y Eucalyptus SP.

Se presenta un método rápido de cromatografía de capa delgada para la determinación cualitativa y cuantitativa de vitamina E (a -tocoferol) en muestras de Pinus caribaea Morelet y Eucaliptus sp. Los extractos etereos se cromatografiaron en placas preelaboradas con silicagel, para lo cual se empleó cloroformo como fase móvil. La cuantificación se realizó por detección densitométrica de las manchas del compuesto mediante un sistema controlado por computadora en los modos de reflexión/absorbancia a una longitud de onda de 280 nm. Este trabajo constituye el primer reporte de la presencia de esta sustancia en Cuba, encontrándose la vitamina E en concentraciones de 177 y 2 400 mg/kg de droga seca en pino y eucalipto respectivamente.

A fast methods of thin layer chromatography for the qualitative and quantitative determination of vitamine E (a -tocopherol) in samples of Pinus caribaea Morelet and Eucalyptus sp. is presented. The ethereal extracts were chromatographied in plates preelaborated with silica gel, for which chloroform was used as a mobile phase. Quantification was carried out by densitometric detection of the compound stains, using a computer controlled system in the modes of reflexion absorbability at a wave longitude of 280 nm. This paper is the first report about the presence of this substance in Cuba. Vitamin E has been found in concentrations of 177 and 2 400 mg/kg of dry drug in pine and eucalyptus respectively.

Tamizaje fitoquímico preliminar de plantas que crecen en Holguín

Se realizaron pruebas químicas preliminares de detección de aminoácidos, saponinas, taninos, esteroles y triterpenos, glicósidos cardiotónicos, fenoles símples, cumarinas y flavonoides (antocianidinas) a 83 partes vegetales de 39 especies, de ellas 34 endémicas, correspondientes a 24 familias de plantas que crecen en Sierra de Nipe, provincia Holguín, para lo cual se utilizó el método establecido por Rondina y Coussio con modificaciones. Se presentan en forma de tablas las familias, géneros y especies ordenadas alfabéticamente, las partes de las plantas investigadas, los resultados químicos obtenidos y las aplicaciones de cada grupo de fitoconstituyentes.

Preliminary chemical tests for detecting aminoacids, saponins, tannins, sterols and triterpenes, cardiotonic glycosides, simple pehnols, cumarins and flavonoids (antocyanidins) were carried out in 83 vegetable parts from 39 species, 34 of them endemics, corresponding to 24 families of plants growing in Sierra de Nipe, province of Holguin. The method established by Rondina and Coussio was used with modifications. Families, genera, and species in alphabetical order, as well as the parts of plants investigated, the chemical results obtained, and the applications of each group of phytoconstituents, are shown in the tables.

Los liposomas en dermatoterapia

It id presented a review of those pharmaceuticals of topical use reported in literature and which have been encapsulated in liposomes aimed at increasing their biological effectiveness in the treatment of different skin diseases, making emphasis on the analysis of the results obtained by diverse authors. History of the application of liposomes in the topical administration of drugs is dealt with, and the methods and techniques used for studying the interaction between liposomes and skin are briefly analyzed. The results obtained so far allow to predict a wide use of the liposomic systems in dermatology in the future, taking into account that the advanced administration systems offer numerous advantages, such as: increase of the local effect with the subsequent reduction of systemic toxicity, versatility to attain a dermic or transdermic system depending on formulation, and a greater cosmetic acceptability, among others.