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Inoculación de trigo (Triticum aestivum, L.) con rizobios adaptados a ecosistemas ganaderos de Alberta, Canadá


 
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 ISSN 0864-0394

BECQUER, C.J et al. Inoculación de trigo (Triticum aestivum, L.) con rizobios adaptados a ecosistemas ganaderos de Alberta, Canadá. []. , 30, 1, pp. 1-1. ISSN 0864-0394.

^les^aSe efectuó un ensayo en invernadero con el objetivo de seleccionar cepas de rizobio inoculadas en trigo (Triticum aestivum, L., var. CDC Teal). Para ello se utilizaron 16 cepas nativas canadienses pertenecientes a Sinorhizobium medicae, así como 12 cepas comerciales. Los inóculos se confeccionaron en medio CLM y fueron agitados hasta alcanzar un título de 106-108 cél./mL. Se utilizaron los métodos estándar para la inoculación de cereales. Se construyó un dendrograma mediante el método de Ward (distancia euclidiana al cuadrado). Se evaluaron las variables: peso seco aéreo (PSA), peso seco raíz (PSR), longitud del tallo (LT), peso seco espigas (PSE) y número de hijos (NH). Se constató un incremento significativo en todas las variables en los tratamientos inoculados con las cepas nativas y las cepas comerciales de rizobio, comparados con el testigo absoluto. En peso seco aéreo, peso seco espigas y peso seco raíz, la mayor parte de los tratamientos no difirieron significativamente del control fertilizado. En longitud del tallo existieron 17 tratamientos superiores estadísticamente con respecto al control fertilizado. Se seleccionaron 10 cepas del total. Los tratamientos seleccionados en el dendrograma constituyeron el 31% para las cepas nativas y el 42% para las comerciales. Se recomienda efectuar ensayos de campo con las cepas seleccionadas inoculadas en cereales de interés económico para Cuba.^len^aA greenhouse experiment was carried out in order to select rhizobium strains inoculated on wheat (Triticum aestivum, L., var. CDC Teal). For that 16 Canadian native strains, belonging to Sinorhizobium medicae, as well as 12 commercial strains, were used. The inocula were prepared in YMB medium and shaken up to obtain a title of 106-108 cells/mL. The standard methods for the inoculation of cereals were used. A dendrogram was constructed by means of the Ward's method (square Euclidian distance). The variables: aerial dry weight (ADW), dry weight of root (DWR), stem length (SL), dry weight of spikes (DWS) and number of shoots (NS) were evaluated. A significant increase was observed in all the variables in the treatments inoculated with the native strains and the commercial strains of rhizobium, as compared to the absolute control. In aerial dry weight, dry weight of spikes and dry weight of root, most of the treatments did not differ significantly from the fertilized control. In stem length there were 17 treatments statistically higher than the fertilized control. Ten strains were selected from the total. The treatments selected in the dendrogram constituted 31% for the native strains and 42% for the commercial strains. Field trials with the strains selected inoculated on cereals of economic interest for Cuba are recommended.

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