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Agentes fungosos asociados a síntomas de enfermedades en plántulas de Moringa oleifera Lamarck


 
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Pastos y Forrajes

 ISSN 0864-0394

LEZCANO, J. C; ALONSO, O; TRUJILLO, Marialys    MARTINEZ, E. Agentes fungosos asociados a síntomas de enfermedades en plántulas de Moringa oleifera Lamarck. []. , 37, 2, pp. 166-172. ISSN 0864-0394.

^les^aEl objetivo de esta investigación fue diagnosticar e identificar los patógenos asociados a diversos síntomas de enfermedades en plántulas de Moringa oleifera, específicamente de las procedencias Supergenius y Nicaragua. El material vegetal (hojas y tallos con manchas, necrosis y clorosis foliar, y marchitez de las plántulas) se colectó en el organopónico de la Estación Experimental de Pastos y Forrajes Indio Hatuey y se desinfectó, para proceder al aislamiento e identificación de los agentes causales. Esto se realizó a partir de la caracterización cultural y morfológica de los aislamientos, que fueron cultivados en placas Petri de cristal esterilizadas, de 9 cm de diámetro con los medios agar papa y dextrosa y agar hojas de clavel, y con el empleo de claves taxonómicas. El diagnóstico presuntivo permitió identificar la presencia de Colletotrichum dematium (Pers.) Grove y Fusarium solani (Mart.) Sacc., en las plántulas afectadas. El primero se vinculó fundamentalmente con síntomas como clorosis, necrosis del tallo y manchas foliares pequeñas y redondeadas, con el centro pardo claro y el borde pardo oscuro, localizadas en la haz y el envés, y dispersas sobre la lámina foliar. Mientras que el segundo se relacionó con manchas y necrosis del tallo y marchitez y muerte de las plántulas de M. oleifera.^len^aThe objective of this study was to diagnose and identify the pathogens associated to diverse disease symptoms in Moringa oleifera seedlings, specifically in the provenances Supergenius and Nicaragua. The plant material (leaves and stems with spots, necrosis and leaf chlorosis and seedling wilting) was collected at the organoponic garden of the Pasture and Forage Research Station Indio Hatuey and was disinfected, to isolate and identify the causative agents. This was done from the cultural and morphological characterization of the isolations, which were cultivated on glass Petri dishes sterilized, of 9 cm diameter with the media potato and dextrose agar and carnation leaf agar, and using taxonomic keys. The presumptive diagnosis allowed to identify the presence of Colletotrichum dematium (Pers.) Grove and Fusarium solani (Mart.) Sacc., in the affected seedlings. The former was mainly linked to such symptoms as chlorosis, stem necrosis and leaf spots small and rounded , light brown at the center and dark brown at the edge, located on the face and the back, and spread over the leaf lamina; while the latter was related to stem spots and necrosis and wilting and death of the M. oleifera seedlings.

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