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Densidad superficial nuclear de hepatocitos de hígados sometidos a un factor estimulante humoral en ratones


 
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Revista Archivo Médico de Camagüey

 ISSN 1025-0255

CONCEPCION OSORIO, Marielín; DIAZ ROJAS, Pedro A.    RODRIGUEZ AMADOR, Tania. Superficial nuclear density of hepatocytes of livers subjected to a humoral stimulating factor in mice. []. , 18, 5, pp. 519-531. ISSN 1025-0255.

Background: hepatic regeneration is the basic answer of the liver to various internal and external stimuli. It is multifactorial and induced and originates sequential changes in the gene expression, the structure and organization of hepatic cells. Objective: to analyze the behaviour of the superficial density of the nuclei of the hepatocytes according to the position they have in the areas of the middle hepatic lobes of mice subjected to regenerative cytosol. Method: the superficial density of the nuclei of the hepatocytes was studied with the use of morphometry in 14 middle hepatic lobes of mice subjected to a dose of regenerative liver cytosol at the beginning of the experiment. The samples were taken every 12 hours during eight days. The cut was divided into proximal, medial and distal areas to the hilum, where the determinations were made. Results: the average values and their standard deviations in the areas were: proximal 701,23 ± 135,83 (μm2.103), medial 707,48 ± 46,87 (μm2.103) y distal 682,48 ± 125,21 (μm2.103). When comparing the results through the analysis of variance, it could be seen that there are not differences among the averages in the three areas. When comparing the initial stage with the final stage of the experiment major differences (p ≤ 0,05) could be seen in the same areas, predominating the proximal and distal area. Conclusions: in the three areas of the lobe the behaviour of the superficial density of the nuclei of the hepatocytes is similar, with a tendency to increase at the end of the experiment. This could be corroborated with the application of the Student’s t- test. In spite of the fact that there were major differences between the values obtained in the first part and the final part of the experiment in all the areas, the most significant increases occurred in the proximal and distal areas.

: MEASUREMENTS, METHODS AND THEORIES; HEPATOCYTES [cytology]; LIVER REGENERATION; ANIMALS; EPIDEMIOLOGY, EXPERIMENTAL.

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