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Revista Habanera de Ciencias Médicas
On-line version ISSN 1729-519X
Abstract
SOLANA RODRIGUEZ, Jessica Ocalia et al. Characterization of hyperimmune anti-human serum produced in sheep. Rev haban cienc méd [online]. 2020, vol.19, n.1, pp. 30-39. Epub Feb 01, 2020. ISSN 1729-519X.
Introduction:
Immunoelectrophoresis is a precipitation technique that allows the characterization of complex biological samples. The Immunology Department of the Institute of Basic and Pre-Clinical Sciences “Victoria de Girón” has a hyperimmune antiserum obtained by immunization of sheep against human serum total proteins and it also has an anti-human IgA antiserum obtained from human colostrum.
Objective:
The aim of this study was to identify IgG, IgM and IgA in human serum and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.
Material and Methods:
An observational descriptive cross-sectional study was conducted from November 2017 to June 2018. Immunoelectrophoresis of normal human serum was performed using hyperimmune antiserum.
Results:
These procedures allowed to identify IgG, IgM and IgA in addition to albumin and other protein fractions and to determine response to anti-human IgM in human colostral IgA with antiserum obtained in sheep.
Conclusions:
This work allowed us to identify and determine significant anti-class responses of immunoglobulins in the sample studied.
Keywords : Immunoelectrophoresis; hyperimmune antiserum; immunoglobulin A; immunoglobulin G; immunoglobulin M.