Resumen

FERNANDEZ, Kalyanne. Mycorrhization in vitro and in vivo of potato plantlets (Solanum tuberosum var. Alpha). cultrop [online]. 2010, vol.31, n.2, pp. 00-00. ISSN 0258-5936.

The artificial conditions under which micro-propagated plants develop make them more susceptible to drought when transplanted. Arbuscular mycorrhizal fungi contribute to plant growth and survival by reducing the stress associated to nutrition, water relations, soil structure, pH, salts, toxic metals and pathogens, so that they are considered extremely useful as inoculants during the different stages of plant micropropagation. Taking these aspects into account, this work was developed to evaluate the mycorrhizal inoculation feasibility during in vitro and in vivo stages of potato (Solanum tuberosum L var. Alfa) crop micropropagation process. Therefore, mycorrhizal inoculation was studied on potato plants under in vitro growing conditions, with different culture media (MS; M and a new medium N), with a randomized complete design (RCD) and potato plants inoculated, when transplanted to adaptation stage, by three single Glomus species and two concentrates of AMF species (forest and desert) on two different growth substrates using a bifactorial arranged RCD. Positive effects were obtained on in vitro plants inoculated in the M medium, even though it is evident to look for new nutritionally-balanced media that guarantee both plant growth and an efficient mycorrhizal establishment. Besides, at the adaptation stage, there was a highly positive strain inoculation response, showing a differentiated behaviour according to the substrates, which was appreciated in all variables studied (nutritional, fungal and physiological), so proving the feasibility of using such microorganisms at this potato micropropagation stage

Palabras clave : arbuscular mycorrhizae; inoculation; micropropagation; adaptation; Solanum tuberosum; culture media.

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