Resumen

ALVARENGA VENUTOLO, Silvana. Mass microprogation of Stevia rebaudiana Bertoni in temporary immersion systems. cultrop [online]. 2015, vol.36, n.3, pp. 50-57. ISSN 1819-4087.

Stevia rebaudiana Bertoni, Asteraceae, is a natural sweetener called “Stevia”. Its properties came from the diterpenoid glycosides presented in the leaves: a stevioside and rebaudisioside. The percentage of seed germination of S. rebaudiana is very low and the plants produced are heterogeneous, so it is not suitable for mass propagation in the field.The tissue culture in temporary immersion systems, is an effective tool for micropropagation, since it increases the multiplication coefficient and produces the improvement in the quality of in vitro regenerated plants. This research determined the efficieness of propagation process using temporary immersion devices for scaling up the mass production of Stevia rebaudiana, with the use of liquid media in automated temporary immersion system (RITA^®) and twin flasks system (BIT), compared with semisolid culture. Most of the treatments involving temporary immersion systems (BIT y RITA^®), produced green plants and vigorous plants, with low levels of hyperhydricity. The highest average of regenerated leaves and shoots was obtained in the RITA^® system, with 10 minutes of immersion every 8 hours. It was determined also that 10 minutes on inmersion each 12 hour with the BIT produced vigorous plants, with an increased length, fresh and dry weight mass. The use of in vitro culture in temporary immersion bioreactors (RITA^® and BIT), offers many advantages in the process of scaling the production of this kind of commercial plant, related to connentional propagation system and encouraging the process of acclimatization

Palabras clave : sweetener; in vitro; culture; bioreactors; propagation; Stevia.

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