Resumen

MONTES-DE GODOY, María Elena. Micropropagation and molecular characterization of a coffee variety (Coffea arabica) resistant to rust (Hemileia vastatrix). cultrop [online]. 2019, vol.40, n.2  Epub 01-Jun-2019. ISSN 1819-4087.

In Santa Ana, Department of El Salvador, there is an accession of coffee derived from Bourbon, which has been sheltered for several generations, enjoying resistance to this disease and maintaining cup quality. The objectives of the work were to establish a micropropagation protocol for this variety, as well as to perform the molecular characterization of the vitroplants obtained. Mature seeds were used, disinfected and introduced into a sterile solution of boric acid and cysteine. The culture medium was an MS (pH 5.7) with 0.5 mg L-1 of 6- BAP. For multiplication, MS was used with the macrosalts at half the initial concentration, supplemented with 1.5 mg L-1 of 6-BAP and 30 g L-1 of sucrose. The multiplication rates varied between 2.1 and 4.5 with statistical differences. For the phase of development and rooting the MS culture medium was used with two treatments: one, without hormone with the macrosalts at half the concentration, and another, of the same formulation plus 2.5 mg L-1 of ANA and 10 g L -1 of sucrose; both kept in darkness for 14 days. For the indicators of number and length of roots in the phase of development and rooting, the formulation medium MS without hormone (T1), presented statistical differences, so it is proposed for the propagation of this accession of coffee. The statistical analysis was Analysis of Variance, with comparison of means by Tukey. For the determination of the genetic fingerprint, microsatellite markers or repeated simple sequences (SSRs) were used; establishing that all the resulting plants have the same genetic profile.

Palabras clave : climate change; genetic stability; microsatellites; resistance.

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