Resumen

SAVON, CLARA et al. Normalización de un ensayo de ultramicroELISA para la detección de anticuerpos IgG al virus sincitial respiratorio. Rev Cubana Med Trop [online]. 1996, vol.48, n.3, pp. 161-162. ISSN 1561-3054.

An ultramicroELISA assay of double antibody for the detection of IgG antibodies to the respiratory syncytial virus (RSV) wasstandardized. It was used a RVS antiprotein F monoclonal antibody produced by the Genetic Engineering and Biotechnology Center (GEBC) in Havana. The use of this antibody allowed to include crude antigenic preparations instead of purified fractions, which caused a significant reduction of the reactivity obtained with the antigen control. The assay conditions were determined by crossed titration. It was obtained a sensitivity of 97.2 %, a coincidence of 91 %, and a specificity of 83.3 % of the UMELISA as regards the complement fixation. The results may be qualitatively expressed or by antibody titres using only one serum dillution (1:40) and a pattern curve.

Palabras clave : ENZYME-LINKED IMMUNOSORBENT ASSAY [standards]; RESPIRATORY SYNCYTIAL VIRUS, HUMAN [immunology]; IGG [blood]; ANTIBODIES, ANTIIDIOTYPIC [BLOOD]; ANTIBODIES, MONOCLONAL [immunology].