Resumen

PEREZ MACHIN, Maykel et al. Validation of an in vivo method to assess the diuretic activity. Rev Cubana Invest Bioméd [online]. 2011, vol.30, n.3, pp. 332-344. ISSN 0864-0300.

To supply a in vivo pharmacological model to determine the diuretic activity of medicinal plants, aqueous extracts were prepared from dry drug of 8 medicinal plants with diuretic activity attributed by the Cuban traditional medicine, but there was an experimental validation. Eighty Sprague-Dawley eight male rats were random distributed at a rate of eight rats by group: positive controls ( 20 mg/kg furosemide and 10 mg/kg hydrochlorothiazide); negative control (NaCI 0.9 %) and 8 groups treated with aqueous extracts from plants supplied by oral route at doses of 400 mg/kg, on the base of determination of total solids. Dose was completed with physiological saline solution to achieve a hydrosaline overload with a total volume of constant administration of 40 mL/kg of weight lives. Rats were placed in metabolic cages measuring the volumes of urine extracted at ½, 1, 2, 3, 4, 5 and 6 h post-administration and the electrolytes concentrations (Na⁺ and K⁺) in total urine collected at 24 h. It was noted that all the treated groups increased the urine volume in relation to negative control group. Urinary excretion, diuretic action and activity were higher in experimental groups: Persea Americana Miller (similar to Furosemide) and Cassia alata L, Zanthoxylum fagara L. (similar to thiazides).

Palabras clave : Diuretics; preclinical; medicinal plants; rats.

        · resumen en Español     · texto en Español     · Español ( pdf )