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Revista Cubana de Química
versión On-line ISSN 2224-5421
Resumen
MENDEZ-RODRIGUEZ, Daniel et al. Chemical composition and antioxidant activity of Coccoloba cowellii Britton. Rev Cub Quim [online]. 2019, vol.31, n.2, pp. 185-198. ISSN 2224-5421.
The qualitative and quantitative chemical composition (total phenolics, tannins and flavonoids contents), as well as the antioxidant activity of an ethanolic extract of the leaves of Coccoloba cowellii (Polygonaceae) was determined in this study. The extract was prepared by dynamic ultrasound-assisted extraction using firstly petroleum ether:ethyl ether (1:1, v/v) and therefore ethanol:water (8:2, v/v). Quantitative chemical composition (total phenolics, tannins and flavonoids content) was determined using spectroscopic techniques, while the antioxidant activity was determined by means of the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing power tests. The ethanolic extract showed significant antioxidant activity in both assays, with values of 34,01 % ± 4,03 % in the DPPH and reduced Fe3+ ferricyanide complex to the ferrous form (Fe2+), similar to the ascorbic acid used as standard in both assays. The antioxidant activity of the extract is consistent with the total phenolic, tannins and flavonoid contents of the extract.
Palabras clave : total phenolics; tannins; flavonoids; antioxidant activity; dynamic ultrasound-assisted extraction.