Resumen

THEODORIDIS, Dimitrios; BOHMER, Jan; HOMUTH, Matthias  y  STRUTZBERG-MINDE, Katrin. Development of a novel ELISA for serodiagnosis of Leptospirosis and additional detection of pathogenic Leptospira by polymerase chain reaction for veterinary routine diagnostics. Rev Cubana Med Trop [online]. 2005, vol.57, n.1, pp. 49-50. ISSN 1561-3054.

A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.

Palabras clave : Leptospirosis; leptospira; ELISA; PCR; diagnosis.

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