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Revista Cubana de Medicina Tropical
versión On-line ISSN 1561-3054
Resumen
CORTES, Fabián M.; GOMEZ, Sergio Y. y OCAZIONEZ, Raquel E.. Subtypes of dengue virus serotypes 2, 3 and 4 isolated in Santander District, Colombia. Rev Cubana Med Trop [online]. 2007, vol.59, n.3, pp. 0-0. ISSN 1561-3054.
Virus serotypes 2, 3 and 4 that had circulated in Santander District, Colombia in the period 1998-2004 were analyzed. Identifying the subtype of a dengue virus serotype is a useful tool for surveillance of severe risk factors because the strain potential to cause hemorrhagic dengue makes the difference among them. Simultaneous sequence amplification technique known as restriction site specific-polymerase chain reaction (RSS-PCR) was used to determine the subtype by comparing the electrophoretic pattern of the local isolate to the reference virus. Virus serotype 2 corresponded to subtype A similar to the one isolated in Thailand (1996) and to the other isolated in Porto Rico (1986); virus serotypes 3 were of subtype C like the virus found in Sri Lanka (1990), Honduras (1995) and Porto Rico (2000); virus serotypes 4 were a variant of subtype B similar to a virus from Porto Rico (1987) and to another virus from Tahiti (1985). The study confirmed the presence in Colombia of dengue virus subtypes circulating now in the Americas.
Palabras clave : Genotype; dengue; serotype; flavivirus; RSS-PCR.