Resumen

ESCOBAR-PEREZ, Xiomara et al. Implementation of tissue microarrays technique for cancer research in Cuba. Vaccimonitor [online]. 2015, vol.24, n.1, pp. 45-51. ISSN 1025-0298.

The tissue microarray (TMA) technique is based on making cylindrical cores from paraffin donor blocks and transfer to a single recipient block. The TMA has revolutionized the field of pathology for the possibility to evaluate multiple samples in one slide. There is no precedent of this subject in Cuba, so the objective of this research was to implement the TMA technique. The concordance of the results obtained by complete section and the TMA were evaluated for this purpose, in the evaluation of the estrogen receptors (ER), progesterone (PR) and epidermal growth factor type 2 (HER2) in samples of breast cancer. Forty-five paraffin-embedded samples from women diagnosed with breast cancer at the Institute of Oncology in 2012 were studied. Two TMA blocks were constructed, and subsequently the expression of markers ER, PR and HER2 was determined by immunohistochemistry, in the complete section of tissue and in the TMA. Kappa index was used for concordance analysis. A good concordance was obtained for all three markers (ER k=0.8272; PR k=0.793 and HER2 k=0.716). This study constitutes the first report on the TMA technique in Cuba and shows that it is a valuable tool, suggesting its potential use in translational research and clinical trials on vaccines. The tissue microarray (TMA) technique is based on making cylindrical cores from paraffin donor blocks and transfer to a single recipient block. The TMA has revolutionized the field of pathology for the possibility to evaluate multiple samples in one slide. There is no precedent of this subject in Cuba, so the objective of this research was to implement the TMA technique. The concordance of the results obtained by complete section and the TMA were evaluated for this purpose, in the evaluation of the estrogen receptors (ER), progesterone (PR) and epidermal growth factor type 2 (HER2) in samples of breast cancer. Forty-five paraffin-embedded samples from women diagnosed with breast cancer at the Institute of Oncology in 2012 were studied. Two TMA blocks were constructed, and subsequently the expression of markers ER, PR and HER2 was determined by immunohistochemistry, in the complete section of tissue and in the TMA. Kappa index was used for concordance analysis. A good concordance was obtained for all three markers (ER k=0.8272; PR k=0.793 and HER2 k=0.716). This study constitutes the first report on the TMA technique in Cuba and shows that it is a valuable tool, suggesting its potential use in translational research and clinical trials on vaccines. <p></p>

Palabras clave : tissue microarray; immunohistochemistry; breast cancer.

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