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Vaccimonitor
versión impresa ISSN 1025-028X
Resumen
DEL PUERTO, Carmen Alina et al. Improvements in the potency assay of Bordetella pertussis. Vaccimonitor [online]. 2013, vol.22, n.2, pp. 40-42. ISSN 1025-028X.
Potency of pertussis component, present in DPT-vax, is evaluated by Kendrick assay. Strain Bordetella pertussis 18323 is recommended by WHO, which should be stored in a way that ensures its viability, purity and virulence. As part of the assay, the concentration of a strain suspension should be calculated, which opacity is compared at first sight with the Fifth Reference International Preparation of Opacity. This method is very inexact and frequently leads to errors. A seed lot from Bordetella. pertussis 18323 was elaborated and characterized in this study and the concentration of the strain using a calibration curve was calculated. The lot was used in eight potency assays and they complied with established validity criterion
Palabras clave : Bordetella pertussis; potency assays; challenge suspension.