Resumen

CUBA-ROMERO, Yaritza; GAINZA-SANTOS, Noemi; SALTAREN-COBAS, Alfredo  y  NARANJO-MEDINA, Mariela. Evaluation of immunogenicity and protective homologous capacity of a tetravalent Leptospira vaccine candidate, for veterinary use, including serogroup Ballum. Vaccimonitor [online]. 2016, vol.25, n.3, pp. 0-0. ISSN 1025-0298.

Immunogenicity is an important parameter for studies of immunogens. The humoral immune response against leptospirosis is vital for resistance to the infection; hence this paper is proposed to evaluate the immunogenicity and protective homologous capacity of a tetravalent candidate that includes serogroup Ballum in its formulation. The strain 245-12 classified as L. borgpetersenii serovar Ballum was used in this paper, isolated from a confirmed case of leptospirosis. Five (5) batches of a tetravalent vaccine preparation were formulated with this strain previously characterized from the point of view of its virulence, which also contains the strains Canicola, Icterohaemorrhagiae and Pomona contained in the Polyvalent-Leptospira vaccine for veterinary use. The immunogenicity of this preparation was assessed by micro agglutination and homologous protection capacity in Golden Syrian Hamster before the challenge with 100 LD₅₀. Animals were immunized with two doses of vaccine (0.1 mL) with an interval of 15 days; the challenge was carried out 14 days after the immunization schedule was ended. The results were compared with those obtained with the Polyvalent-Leptospira vaccine. All the batches of the tetravalent preparation formulated complied satisfactorily with the quality controls. The batches formulated in this study showed a significant immunogenicity and homologous protection capacity against serogroup Ballum and they were able to eliminate the carrier state in the immunized animals. This paper, for the first time sets the bases for novel vaccine formulations, in animals, containing the serogroup Ballum.

Palabras clave : Leptospira; vaccines; immunogenicity; Ballum.

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