Resumen

CARDOZO BURGOS, Carolina; SILVA AGUILAR, Bernardo; SALAZAR YEPES, Mauricio  y  MORALES OSORIO, Juan Gonzalo. Genetic diversity of Ralstonia solanacearum isolates from three Colombian regions. Rev. Protección Veg. [online]. 2015, vol.30, n.3, pp. 213-224. ISSN 2224-4697.

The present work was performed to analyze the genetic diversity of the R. solanacearum species complex in three Colombian regions where economically important host crops are grown. Sixty nine isolates were collected from different hosts and soils. The genetic diversity was determined using Random Amplified Microsatellites (RAMs) as molecular markers . The data were analyzed by the Nei and Li coefficient, Multiple Correspondence Analysis (MCA), and the Shannon index. The Nei and LI coefficient values found at a similarity level of 0.76% allowed grouping the isolates into three major divisions.. The MCA showed a similarity coefficient of 67% within 10 groups and 62% between groups. The Genetic differentiation coefficient (Gst) showed a value of 0.13 indicating that the genetic diversity was higher within each group of isolates than when the different groups were compared one another. The Shannon index value corresponded with the other calculated indices, indicating that the isolates within each group showed a higher genetic diversity than that observed between the different groups. The Inter-population Gst value (0.0514) suggested a moderate genetic differentiation in the population. The results suggested a high gene flow between populations.

Palabras clave : Ralstonia solanacearum; genetic diversity; RAMs.

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