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Pastos y Forrajes
versão On-line ISSN 2078-8452
Resumo
FUENTES-ZALDIVAR, Maykelin; SOCA-PEREZ, Mildrey; ARECE-GARCIA, Javier e HERNANDEZ-RODRIGUEZ, Yuniel. In vitro acaricidal activity of the oil from Jatropha curcas L. in engorged females of Rhipicephalus (Boophilus) microplus. Pastos y Forrajes [online]. 2017, vol.40, n.1, pp. 49-54. ISSN 2078-8452.
In order to evaluate the in vitro efficacy of the oil from the Jatropha curcas L. fruit in engorged females of Rhipicephalus (Boophilus) microplus (certified Cayo Coco strain), an immersion test of adult ticks was conducted, based on a completely randomized design and nine treatments: negative control of Tween-80 at 5, 10, 15 and 25 %; positive control with Butox® (Deltametrina) and different concentrations of J. curcas oil: 5, 10, 15 and 25 %. The hatching reduction, estimated reproduction and acaricidal efficacy were determined. Through a variance analysis the efficacy of the treatments was determined and by a Probit analysis, the mean effective concentration (EC50) and maximum effective concentration (EC90). The oil significantly inhibited (p< 0,05) the egg hatching percentage, with values of 9,74; 11,40; 11,95 and 13,75 % for the concentrations of 5, 10, 15 and 25 %, respectively. No significant effects were observed in the egg weight, but they were appreciated in the estimated reproduction (p< 0,05) for the experimental treatments with regards to the negative control. The oil of the J. curcas fruit showed marked efficacy as acaricide in the control of R. (B.) microplus, with values higher than 91 %; the best results were found with the lowest concentration (5 %). The dose-response curve showed an EC50 of 88,90 mg/mL, while the EC90 was 37,22 mg/mL. It is concluded that the J. curcas oil has in vitro acaricidal activity.
Palavras-chave : hatching; ticks; eggs.