Resumo

SOTO, Yudira et al. Aplicación de la técnica de reacción en cadena de la polimerasa para la detección de secuencias de Papillomavirus humano. Rev Cubana Med Trop [online]. 1998, vol.50, n.3, pp. 191-198. ISSN 1561-3054.

The polymerase chain reaction technique was applied to detect sequences of human Papillomavirus (HPV) by controls of cellular lines of cervical cancer and of tissues obtained through biopsy with a HPV-positive clinical diagnosis. A set of consensus oligonucleotides, which are complementary to a highly conserved region within the open reading frame El of the viral genome of HPV affecting the cervical mucosa, was used. With these primers it was possible to amplify DNA sequences corresponding to HPV 6 and 11, considered in the low risk group, and to HPV 16, 18, 31 and 33, included in the high risk group. The study of the sensitivity of the amplification technique showed a level of detection of 3,5 viral particles per each cellular diploid genome.

Palavras-chave : PAPILLOMAVIRUS, HUMAN [genetics]; POLYMERASE CHAIN REACTION [methods]; SEQUENCE ANALYSIS DNA [methods]; GENOME, VIRAL; DNA, VIRAL [genetics].

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