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Revista Cubana de Medicina Tropical
versão On-line ISSN 1561-3054
Resumo
TORANO PERAZA, Gilda T; RODRIGUEZ ACEBO, Werner e MARTINEZ MACHIN, Gerardo. Evaluación del polisacárido capsular tipo específico de Cryptococcus neoformans como inmunógeno y antígeno control positivo. Rev Cubana Med Trop [online]. 1998, vol.50, n.3, pp. 207-208. ISSN 1561-3054.
The specific type of capsular polysaccharide of an autoctonous strain of Cryptococcus neoformans var. neoformans was obtained by using the method of selective precipitation with hexadimethrine bromide. The capsular polysaccharide was matched to lambs erythrocytes and it was used as an immunogen in rabbits. Antibody titres of up to 1:32 were attained. Doble serial dilutions of the capsular polysaccharide were evaluated as positive control antigen by contraimmunoelectrophoresis, latex and ELISA techniques, showing biological activity.
Palavras-chave : CRYPTOCOCCUS NEOFORMANS [immunology]; POLYSACCHARIDES [immunology]; ANTIBODIES, FUNGAL; ANTIGENS, FUNGAL.