Resumo

RODRIGUEZ, María Magdalena et al. Mode of inheritance of temephos (Abate) resistance in Aedes aegypti (Diptera: Culicidae) from Cuba. Rev Cubana Med Trop [online]. 2006, vol.58, n.2, pp. 0-0. ISSN 1561-3054.

A study on the mode of inheritance of temephos resistance was conducted using a temephos resistant Aedes aegypti reference strain (SAN-F6) with a value of resistance factor of 200x, compared with the insecticides susceptible Aedes aegypti strain (ROCKEFELLER). Genetic crossings were performed between temephos resistant and susceptible strains. An F1 crossing was attained. The females of this F1 crossing were crossed with males from the ROCKEFELLER strain (retrocrossing), and it was found that the temephos resistance was inherited in a semidominant way and as a monofactorial trait. The activity of Est-A4 observed in the polyacrylamide gel electrophoresis and measured by biochemical assays was higher in the strain resistant to temephos (SAN-F6), lower in the susceptible strain (ROCKEFELLER), and intermediate in the crossing of these two strains. A lower effect of the resistant parental strain was observed in the retrocrossing, both in the mortality with temephos and in the activity of Est. A4. These results suggest that the esterase activity may also be inherited, as well as the resistance to temephos, as a semidominant character.

Palavras-chave : Aedes aegypti; resistance; inheritance mode; temephos; esterase; glutathione transferase.

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