Resumo

ESPINOSA-REYES, Ángel; SILVA-PUPO, Juan José; BAHI-AREVICH, Marisel  e  ROMERO-CABRERA, Dariannis. Influence of in vitro plant size and substrate type on the acclimatization of Morus alba L. Pastos y Forrajes [online]. 2019, vol.42, n.1, pp. 23-29. ISSN 2078-8452.

The objective of this study was to evaluate the influence of size and substrate type on the acclimatization of in vitro Morus alba L. plants. For such purpose, three trials were conducted: in the first one in vitro plants were evaluated grouped according to their sizes, which constituted the treatments (T1: 1,5-2,5 cm; T2: 2,6-3,5 cm and T3: higher than 3,5 cm); in the second one, plants with a length between 2,5 and 3,0 cm were selected and three formulations of substrate mixtures were evaluated: T1: soil (70 %)-cattle manure (20 %)-zeolite (10 %), T2: soil (45 %)-cattle manure (45 %)-zeolite (10 %), T3: soil (90 %)-zeolite (10 %); and in the third trial the substrate type was evaluated on the in vitro plant growth under nursery conditions, with the same treatments. A simple variance analysis was carried out, and Tukey’s multiple range comparison test was applied for p ≤ 0,05. The statistical package Infostat (2017) on Windows® was used. The increases in sprout length and survival (93,6 %) were significantly higher when in vitro plants from 1,5 to 2,5 cm long were used. Using the substrate mixtures T1 and T2 a survival higher than 80 % was obtained; just like higher length, number of leaves and leaf size in the in vitro plants in the initial stage of acclimatization and under nursery conditions. It is concluded that with the use of in vitro plants of size between 1,5 and 2,5 cm and the substrates of treatments T1 and T2 during acclimatization, high survival and higher growth and development of the M. alba plants were achieved.

Palavras-chave : growth; tissue culture; survival; in vitro plants.

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