Resumo

HERNANDEZ, P.J.E et al. Effect of cryopreservation of sheep semen related to its viability and acrosomal status. Rev Salud Anim. [online]. 2012, vol.34, n.2, pp. 78-83. ISSN 0253-570X.

In the present research, the effects of cryopreservation on the viability and acrosomal status of sheep sperms were analyzed. Forty five ejaculates were obtained by the artificial vagina method for analysis, evaluating in fresh semen the following characteristics: volume (Vol), progressive motility (PM), viability percentage (Viab), normal morphology (NM), spermatic concentration (Concentr); as well as its viability and acrosomal status, The last two parameters were evaluated staining with fluorescein isothiocyanate conjugated with Arachis hypogaea lectin and propidium iodide (FITC-PNA/IP), in which there were different patterns of staining: live sperms without acrosomal reaction (VsRA), live with acrosomal reaction (VcRA), dead without acrosomal reaction (MsRA) and dead with acrosomal reaction (McRA), obtaining averages of: 1.2±0.3 ml, 88.0±3.4%, 91.4±3.8%, 94.2±2.9%, 1768.1±5.5 x106/ml, 76.7±5.5%, 7.6±2.7%, 11.2±4.1% and 4.5±2.6% respectively. Semen was frozen in a commercial diluent (Triladyl(r)) and packaged in 0.5 ml straws at a concentration of 150x106/ml in liquid nitrogen for 8 days, thawed at 37°C for 60 seconds. In post-thawed semen evaluation, the following values were determined: PM= 37.4±5.3%, Viab= 67.5±4.7, NM= 79.5±5.7, VsRA= 26.9±7.3%, VcRA= 29.2±6.4%, MsRA= 27.7±7% y McRA= 15.9±6.2%, obtaining statistically significant differences (p<0.05) caused by cryopreservation effects. It is concluded that although viability and acrosomal status of sperm are affected by cryopreservation, live sperm without acrosomal reaction can be used in assisted reproduction techniques.

Palavras-chave : cryopreservation; viability; acrosomal reaction; FITC-PNA/IP; sheep semen.

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