Resumo

HERNANDEZ, P.J.E et al. Effect of cryopreservation on viability and the acrosomal state of new zeland rabbit (Oryctolagus cuniculus) semen. Rev Salud Anim. [online]. 2012, vol.34, n.3, pp. 188-191. ISSN 0253-570X.

The effect of cryopreservation on the viability and the acrosomal state of spermatozoa of New Zeeland rabbits (Oryctolagus cuniculus) was studied. Forty six ejaculates, obtained with an artificial vagina, were used to determine the percentages of viability and mortality with and without acrosomal reaction (VsRA, VcRA, MsRA and McRA, respectively) in fresh and post-thawed semen by staining with fluorescein isothiocianato conjugated with lectine from Arachis Hypogaea and propidium iodide (FITC-PNA/IP). The values obtained in fresh semen were 73.6+4.9%, 5.5+2.4%, 15.2+3.7% and 5.6+2.3%, respectively, and 40.0+4.8%, 10.4+3.1%, 38.1+6.1 y 11.5+3.6, respectively, in post-thawed semen. Statistically significant differences (p<0.05) were determined between the spermatic characteristics in fresh and post-thawed semen, which were caused by the effects of cryopreservation. It is concluded that, even when the viability and the acrosomal state of the spermatozoa are affected by cryopreservation, a sufficient quantity of spermatozoa remains alive and without acrosomal reaction capable to fulfill the fertilization.

Palavras-chave : cryopreservation; viability; acrosomal reaction l; FITC-PNA/IP; Oryctolagus cuniculus.

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