Resumo

MARTINEZ MARRERO, Nadia et al. Genetic relationships between Cuban and Brazilian buffaloes (Bubalus bubalis) by microsatellite markers. Rev Salud Anim. [online]. 2015, vol.37, n.3, pp. 152-163. ISSN 2224-4700.

Researches on genetic relationships among water buffalo populations are useful in supporting conservation decisions and designing of breeding schemes. The aim of this study was to assess the genetic relationships among Cuban Buffalypso/Carabao hybrids and Murrah and Jaffarabadi Brazilian buffaloes using nine cattle microsatellite markers. During the analysis, four loci were excluded due to the presence of null alleles, stuttering and gametic disequilibrium. Mean numbers of alleles and the heterozygosities observed and expected in the three populations were not significantly different. Heterozygosities found were above 60%. All five loci were in Hardy-Weinberg equilibrium. Wright´s index for estimating inbreeding were negative in the three populations. A moderate population differentiation was found among Cuban and Brazilian populations. The AMOVA showed that only 8.3% of the total variation observed occurred among breeds (P<0.05). The multivariate factorial correspondence analysis showed low breed-specific clustering and most of Murrah and Jaffarabadi Brazilian animals plotted overlapping. UPGMA dendrogram obtained from Nei's D_A genetic distance differentiated two clusters, one grouped Cuban buffaloes (bootstrap value 89%), and the other included the Brazilian populations (bootstrap value 100%). Bayesian clustering approach reinforced the previous results. The origin of Buffalypso breed and its genetic management in Cuba could be the reason to the moderate differentiation between Cuban and Brazilian populations. This study provided a preliminary analysis on genetic relationships between Cuban and Brazilian buffaloes. The research must be extended to include a higher number of microsatellite loci and buffalo populations from other geographical regions of each country.

Palavras-chave : Bubalus bubalis; Buffalypso; microsatellite markers; null allele; genetic relationships.

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