Resumo

FERNANDEZ SUAREZ, Kalyanne; FERNANDEZ MARTINI, Félix  e  DECLERCK, Stéphane. Searching of a new culture medium for in vitro mycorrhization of potato (Solanum tuberosum L.) plantlets. cultrop [online]. 2013, vol.34, n.4, pp. 9-19. ISSN 0258-5936.

The culture medium is the key stone to guarantee the establishment of arbuscular mycorrhizal symbiosis on in vitro plants, because it includes organisms with different nutritional requirements. The aim of this investigation was to develop a culture medium, starting from nutritional modifications made to MS medium, in order to allow the in vitro mycorrhization of potato plants with an adequate nutritional state. Two experiments were carry out using three culture media (SRM, MS and ½MS), as well as different nutrients concentrations of MS medium. Potato plantlets, of seven and ten days old, were inoculated in these media with Glomus clarum spores in a partially in vitro autotrophic system. Fungal and potato development were sampled after 20, 30 and 40 days of culture. A differentiated behavior of fungal and potato growth parameters were observed, depending of the culture medium used, been the E medium (¼P - ¼N - ¼K respect to MS) the one where the major values of variables were found. Not differences between inoculated and controls plantlets were found during the experiments.

Palavras-chave : culture medium; in vitro mycorrhization; potato.

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