Resumo

RESIK AGUIRRE, Sonia et al. Aplicación de la enzima SensiScript a un sistema RT-RCP para la obtención de ARN de VIH-1 a partir de muestras de suero almacenadas a -20 oC durante una década. Rev Cubana Med Trop [online]. 2003, vol.55, n.3, pp. 213-216. ISSN 1561-3054.

The efficiency of SensiScript reverse transciptase to obtain useful genetic material in the sequencing of the nucleic acid from HIV-1, starting from sera collected between 1989 and 1998 and kept at suboptimal temperatures, was proved. On using the SensiScript enzyme it was obtained an amplification of the RNA of the HIV-1 in 86.5 % of the studied samples, compared with 20 % on using the AMV-RT enzyme . No amplification was obtained in 13.5 % of the studied samples with any of the 2 enzymes used.

Palavras-chave : REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION [methods]; HIV-1; SEQUENCE ANALYSIS; RNA; ENZYME TESTS.

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