Resumo

HERNANDEZ DIAZ, Patricia; MARTIN GONZALEZ, Odalys; RODRIGUEZ DE PABLOS VELEZ, Yoryelín  e  GANEN BAEZ, Félix A. Study of phytohemagglutinin from red bean (Phaseolus vulgaris). Rev Cubana Hematol Inmunol Hemoter [online]. 1999, vol.15, n.3, pp. 210-214. ISSN 1561-2996.

Raw preparations of phytohemagglutinin (PHA) were obtained from red bean (Phaseolus vulgaris) by 2 methods: aqueous extraction and acid extraction. Better results were obtained with the acid methods as regards the protein concentration and its purity. Hydroxyapatite chromatography was used to separate the isoforms forming the phytohemagglutinin. 5 protein fractions were obtained and functionally evaluated together with the raw preparations through their erythroagglutinating and leukoagglutinating properties. As the ionic force of the medium increased it was observed that the fractions obtained presented a relative decrease of the leukoagglutinating activity as well as a relative rise of the crythroagglutinating activity. The raw preparations and the fractions were electrophoretically evaluated and bands very similar to that of the PHA used as a pattern were obtained

Palavras-chave : PHYTOHEMAGGLUTININS.

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