Resumo

GOMEZ CONSUEGRA, Olimpia; PINON GOMEZ, Mayte  e  MARTINEZ ZUBIAUR, Yamila. Pyramiding TYLCV and TSWV resistance genes in tomato genotypes. Rev. Protección Veg. [online]. 2015, vol.30, n.2, pp. 161-164. ISSN 2224-4697.

The Cuban tomato line LD3 , in which the Ty-1 gene from Solanum chilense LA 1969 was detected, was crossed with an F₁ hybrid carrying the genes Ty-3 from S. chilense LA 2779 and Sw-5 from S. peruvianum. A hundred F₂ plants were transplanted to a ferralitic soil in the field and the F₂ plants individually evaluated in the field with high natural TYLCV-IL (Cu) incidence by using a scale from 0 (no symptom) to 4 (severe stunting). The total DNA extracted from the F₂ plants were analyzed per individual plant by using PCR markers linked to the genes Ty-3 (resistance to TYLCV) and Sw-5 (resistance to TSWV) so that both genes could be detected in a same genotype. Eighty two F₂ plants showed no symptoms under natural TYLCV incidence (0 symptoms) with a productivity ranging from 1.6 to 9.7 kilograms per plant. The Principal Component Analysis (PCA) permitted grouping the different genotypes. Six F₂ plants showed the Ty-3 gene in homozygosis and 12 in heterozygosis; 33 F₂ plants showed the Sw-5 gene according to their DNA analysis. Three F₂ plants (number 65, 68 and 78) showed the Ty-3 and Sw-5 genes in homozygosis besides the Ty-1 gene (resistance to TYLCV) from their female parent. Their productivity ranged from 6.0 to 6.8 kilograms per plant. These are the first Cuban results in which resistance genes for two important diseases in the tomato crop are obtained in a same genotype by the early and simultaneous molecular screening for resistance and the productivity evaluation under field conditions, Both tools, when simultaneously used, are efficient in reducing selection time and space.

Palavras-chave : TYLCV; TSWV; pyrimiding; resistance genes; tomato; genotypes.

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