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Revista Archivo Médico de Camagüey
versão On-line ISSN 1025-0255
Resumo
HERNANDEZ BETANCOURT, Oscar; COMPTE ALBERTO, Oscar; QUESADA LEIVA, Lidice e RODRIGUEZ PARGAS, Ayní. Electrophoretic and chromatographic characterization of the Rhopalurus junceus scorpion venom. AMC [online]. 2009, vol.13, n.6, pp. 0-0. ISSN 1025-0255.
Background: the blue scorpion venom, Rhopalurus junceus is currently marketed with the name of Escozul. This natural product is used in the treatment of different pathologies; however in the revised literature doesn't appear concerning information to the biochemical characterization of the extract of this Cuban specimen. Objective: to make a preliminary biochemical characterization on the crude venom of the Cuban scorpion by means of the employment of two techniques dedicated to these purposes. Method: the discontinuous electrophoresis of proteins was used in polyacrylamide to 15% and the chromatography of high pressure. Results: a diffuse pattern corresponding to inferior size peptides to fourteen KDa was identified, while for HPLC thirteen peaks were determined in the crude venom of this species. Conclusions: the blue scorpion venom presents a similar molecular composition to the one described for other species with an inferior molecular mixture to fourteen KDa.
Palavras-chave : scorpion venoms; chromatography; electrophoresis.