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Revista Cubana de Estomatología
versão On-line ISSN 1561-297X
Resumo
BERNETT ZURITA, Gelen Patricia et al. Método químico simplificado de desmineralización del esmalte dental humano. Rev Cubana Estomatol [online]. 2019, vol.56, n.1 ISSN 1561-297X.
Introduction:
Abridged experimental methods are required to simulate early demineralizing lesions in a controlled and reproducible way.
Objective:
Perform an in vitro evaluation of a simple method of incipient enamel demineralization.
Methods:
Randomized experimental study with a double factorial replication design. Twelve third molars from healthy human subjects were selected for demineralization in a racemic lactic acid solution. Samples were then distributed randomly: Group 1 (G1) (n= 6) lactic acid at pH 2.4 and Group 2 (G2) (n= 6) lactic acid at pH 5.4. Each group was then subdivided (n = 2) to evaluate the effect of the solutions at three exposure times (7, 15 and 30 days) at 37°C. The evaluation used stereomicroscopes, a digital x-rays apparatus with software for the digital analysis of images, and polarization microscopy. An integration of the response indices was formulated and ANOVA was performed.
Results:
Visual, radiographic and histological findings showed that G1 at time 1 through 3 displayed demineralization characterized by extensive loss (80 % to 100 %) of enamel integrity. Visually, G2 at 7 days exhibited opacity and loss of brightness (16 %), with preservation of the surface structure of the enamel.
Conclusions:
It was shown that employing lactic acid for 7 days at pH 5.4 develops a clinical, radiographic and histological injury similar to an early enamel lesion.
Palavras-chave : dental enamel; lactic acid; tooth demineralization; in vitro techniques; dental digital radiography; polarization microscopy.