Resumo

FERNANDEZ REYES, F; HERNANDEZ PICHARDO, J.E  e  ROSALES AVILA, María del Rocio. EFFECT OF THE ADDITION OF FSH AND LH IN MEDIUM ON THE MATURATION AND THE EMBRYONIC DEVELOPMENT in vitro OF OOCYTES OF SOW. Rev Salud Anim. [online]. 2009, vol.31, n.2, pp. 122-128. ISSN 0253-570X.

One thousand twenty five oocytes from sow ovaries, obtained in slaughter houses were recovered. From them, 165 (treatment 1 ) were matured with 075 U.I. of FSH and 0.75 U.I. of LH, and 125 (treatment 2 ) with 1.5 U.I. of FSH and 1.5 U.I. of LH. Maturation was evaluated by fixation with alcohol-acetic acid and stained with acetic orcein. There was a 40% and 39% of maturated oocytes from the treatments 1 and 2 respectively, without significant statistical differences among them. In the two treatments for in vitro fertilization, the maturation of oocytes was evaluated by polar body observation. They were divided into two groups: one having 155maturated with the first treatment and the other having 137 oocytes with the second treatment, which corresponded to a 42.1% and 37.3% respectively . As result of in vitro fertilization, 122 embryos were obtained in their different stages, reaching 76 with the first treatment and 46 with the second one, for a 49% and 33.5% (a different fom b p <0.05) respectively . It is concluded that even though FSA and LH supplement to the maturation medium TCM-199 (in vitro) did not significantly affect such maturation, in the development of embryos, there was a higher percentage of morulas and embryonic development with the use of 0.75U.I. of FSH and 0.75 0.75U.I: of LH.

Palavras-chave : pig; oocyte; embryo; maturation in vitro; fertilization in vitro.

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