Resumo

RODRIGUEZ MEDINA, Majela; BARRERA VALLE, Maritza; ENGELS, Monika  e  ACKERMANN, M. MOLECULAR CHARACTERIZATION OF A Herpesvirus bovino 1 CUBAN STRAIN. Rev Salud Anim. [online]. 2010, vol.32, n.1, pp. 42-47. ISSN 0253-570X.

In Cuba, infections caused by Bovine herpesvirus 1 (BoHV-1) are endemic; however clinical signs are moderate, possibly because the BoHV-1 circulating genotypes are not associated to severe infections but these are not known. In the present study a BoHV-1 strain isolated in Cuba was compared with BoHV-1 strains from other different countries. Viral DNA was analysed by adoption of a clustering system based on restriction enzyme analysis (REA) with HindIII, HpaI, PstI and SfiI, which led to identify the Cuban isolate as a BoHV-1.1.III strain. The Cuban BoHV-1 differed from all the reference strains analysed and displayed a restriction fragment pattern more similar to strains originated from the European continent. The inclusion of these results into the clustering database is a contribution for future studies in tracing back the origin of novel infectious bovine rhinotracheitis (IBR) outbreaks.

Palavras-chave : Bovine herpesvirus 1; infectious bovine rhinotracheitis; restriction enzyme analysis; clustering system.

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