Resumo

ACEVEDO, Ana María et al. DETECTION IN CLINICAL SAMPLE AND IDENTIFICATION OF AVIAN INFECTIOUSBRONCHITIS VIRUS ISOLATES BY A REVERSE TRANSCRIPTION POLYMERASECHAIN REACTION ASSAY. Rev Salud Anim. [online]. 2010, vol.32, n.2, pp. 112-117. ISSN 0253-570X.

Avian infectious bronchitis (AIB) is a viral disease worldwide distributed and it is considered one of the main causes of economic losses for poultry industry because of its characteristic of affecting development of broiler and laying hens. In Cuba, the current diagnosis is carried out by viral isolation and its identification by conventional methods is difficult. In order to identify the possible presence of avian infectious bonchitis virus in cuban isolates a reverse transcription-polymerase chain reaction assay (RT-PCR) specific for this virus was applied. Trachea-lung samples were taken as pooles of three and four birds after each necropsy, evaluating a total of eleven birds. The sequences of AIB virus were detected in two of three pools. In one of these pools the detection from clinical sample was also obtained, a very important aspect for its differentiation with serious diseases with a similar clinical course as Avian influenza and Newcastle disease. The RT-PCR showed a potential in order to be used in the identification of isolates from AIB virus, as well as in the direct examination of clinical samples opening the possibilities of its use in the quick, sensitive and specific detection of this agent in our country an essential aspect in the diagnosis and control of this viral infection.

Palavras-chave : avian infectious bronchitis virus; viral isolation; reverse transcription polymerase chain reaction (RT-PCR).

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