Resumo

ILLNAIT ZARAGOZI, María Teresa; MARTINEZ MACHIN, Gerardo  e  FERNANDEZ ANDREU, Carlos. Obtención de inmunoglobulina G anti-Cryptococcus neoformans. Rev Cubana Med Trop [online]. 1998, vol.50, n.1, pp. 27-30. ISSN 1561-3054.

Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.

Palavras-chave : CRYPTOCOCCUS NEOFORMANS [immunology]; CHROMATOGRAPHY; ION EXCHANGE, AGGLUTINATION TEST [methods]; RABBITS [immunology].

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