Resumo

GUERRA ORDONEZ, Marta. Ensayos farmacológicos in vitro para evaluar actividad antigiardiÁsica. Rev Cubana Farm [online]. 2001, vol.35, n.1, pp. 66-73. ISSN 1561-2988.

A review of the most used in vitro pharmacologic assays to evaluate the antigiardial activity was made. The first tests of susceptibility to drugs lead to a tedious microscopic observation of the effect of the drug on the motility and morphology of the parasite. The method of growth inhibition by visual count has been traditionally used. A further assay of clonal growth eliminates this inconvenient, but it is technically demanded. The radiometric assay, based on the incorporation of titriated thymidine, that of adherence inhibition of the trophozoites and a colorimetric test using tetrazolium salts, which are metabolically reduced in the living cells to give rise to a colored product, are stressed at present. Each of these assays has advantages and disadvantages. The current trend is to develop and combine methods that make possible the obtention of information about the mechanism of action of the drugs.

Palavras-chave : DRUG RESISTANCE; GIARDIASIS [drug therapy]; GIARDIA LAMBLIA [drug effects]; GIARDIA LAMBLIA [isolation & purification]; ANTIPARASITIC AGENT; "IN VITRO"; MICROBIAL SENSITIVITY TESTS.

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