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Revista de Salud Animal
versão impressa ISSN 0253-570X
Resumo
GONZALEZ, Marlén e VEGA, A. HOW TO CROSS THE CELLULAR MEMBRANE?. Rev Salud Anim. [online]. 2007, vol.29, n.1, pp. 8-20. ISSN 0253-570X.
In order to introduce molecules into cells, different useful procedures have been developed in molecular and cellular biology studies. Among them we have: permeabilization, microinjection, cellular transfection, and transduction methods. The selection of the best method depends on the experiment final purpose and the equipment available. In this review we approach the laboratory methods used to introduce molecules into cells with a special emphasis in their advantages and disadvantages.
Palavras-chave : permeabilization; microinjection; cellular transfection; transduction.