Resumo

DIAZ DE ARCE, Heidy  e  PEREZ, L.J.. DETECTION OF PESTIVIRUS BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN AMPLIFICATION OF THE 5' cDNA UNTRANSLATED REGION. Rev Salud Anim. [online]. 2008, vol.30, n.3, pp. 146-151. ISSN 0253-570X.

Pestivirus genus of the family Flaviviridae includes different viral species that cause significant losses to the livestock industry worldwide affecting cattle, sheep, goats and pigs. A rapid, sensitive, and specific detection of these agents is therefore essential for diagnosis and control of the viral infections. An RT-PCR assay was developed and standardized for the specific panpestivirus detection in serum based on a generic pair of primers that amplifies a 119 pb DNA fragment from a highly conserved region in the 5´-UTR RNA genome. The RT-PCR assay showed to be a specific, rapid, sensitive and highly repeatable purpose.

Palavras-chave : diagnosis; pestivirus; PCR.