Resumo

NAPOLES GARCIA, María C et al. Nod factor detection in B. elkanii ICA 8001. Culture medium influence. cultrop [online]. 2014, vol.35, n.1, pp. 92-99. ISSN 0258-5936.

Nodulation factors, synthesized by different species from Rhizobiaceae family, have been described as key signals in leguminous interaction. They constitute morphogens that elicit the nodular program in the plant, allowing the bacteria to enter the roots and showing a positive influence on bacteroids development and nitrogen fixation efficiency. This study is related to the detection, by means of two chromatographic techniques, of Nod factors production by Bradyrhizobium elkanii ICA 8001 strain, cultivated in different culture media, and the biological effect of inocula in the interaction with plant. Both methods demonstrated that the culture medium composition induce in a higher or lower quantity the Nod factors produced by the bacterium. Also, the inoculants from different media elicit a differentiated nodulation on soybean plant.

Palavras-chave : Bradyrhizobium; nodulation; chromatography; legumes.

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