Resumo

BRAVO FARINAS, LAURA et al. Aplicación de la técnica de hibridación en colonias para la identificación de Vibrio cholerae 01 toxigénico. Rev Cubana Med Trop [online]. 1996, vol.48, n.3, pp. 169-170. ISSN 1561-3054.

By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.

Palavras-chave : POLYMERASE CHAIN REACTION [methods]; VIBRIO CHOLERAE [isolation and purification]; CHOLERA TOXIN [genetics]; NUCLEIC ACID HYBRIDIZATION [genetics].