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Revista Cubana de Medicina Tropical

versão On-line ISSN 1561-3054

Resumo

FERNANDEZ-CALIENES, Aymé; HERNANDEZ, Natividad  e  FRAGA, Jorge. Amplificación al azar del ADN de 5 poblaciones cubanas de peces larvívoros del género Rivulus. Rev Cubana Med Trop [online]. 2003, vol.55, n.3, pp. 203-208. ISSN 1561-3054.

DNA from 10 individuals from 5 populations of Rivulus collected in the western region of Cuba was amplified at random. No monomorphic marker resulted from the amplification by using 5 primers, which showed the high genetic variability existing among the fishes of the 5 populations. According to the genetic distance among the individuals, they were divided into 4 groups, for which there are specific genetic markers of RAPD, since they are not observed in the rest of the subjects. These results support the use of RAPD as an efficient tool for the genetic characterization of Cuban fishes of the Rivulus genus.

Palavras-chave : FISHES [genetics]; DNA; GENETIC MARKERS; INSECT BITES AND STINGS [prevention & control]; MOSQUITO CONTROL.

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