Resumo

MENA LOPEZ, Lázaro et al. Assessment of anti-tetanic antibody titration by UMELISA in special donors. Rev Cubana Hematol Inmunol Hemoter [online]. 2001, vol.17, n.3, pp. 194-200. ISSN 1561-2996.

Accuracy and precision of UMELISA kit was evaluated in anti-tetanic IgG dosing. The variability of antibody concentration as a function of time and storage temperature was assessed in 34 special donors of hyperimmune anti-tetanic plasma, immunized with Cuban-made tetanus toxoid vaccine and subjected to donation methods by automated plasmapheresis. Two study groups were chosen according to serum immunoglobulin concentrations (< 10 UI/ml and > 10 UI/ml) and samples were preserved at _30 ^oC and at 2-8 ^oC were used . The statistical tests in the data processing yielded that this technique is accurate and precise ant that the highest fluctuations occur when titers are low and kept at 2-8^ºC temperatures

Palavras-chave : GAMMMAGLOBULINS [immunology]; ANTIBODIES [pharmacology]; TECHNOLOGY, MEDICAL [methods]; BLOOD DONORS; PLASMAPHERESIS [methods].

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