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Revista Cubana de Hematología, Inmunología y Hemoterapia

versão On-line ISSN 1561-2996

Resumo

QUESADA LEYVA, Lidyce et al. Morphological characterization of umbilical cord blood cells. Rev Cubana Hematol Inmunol Hemoter [online]. 2020, vol.36, n.1  Epub 01-Jul-2020. ISSN 1561-2996.

Introduction:

In recent years, the umbilical cord has become an element of interest for regenerative medicine, based on its importance as a source of stem cells and hematopoietic progenitors.

Objective:

To characterize the morphology of umbilical cord blood for regenerative therapy in newborns from Ana Betancourt de Mora Gyneco-obstetric University Hospital.

Methods:

Observational, analytical and cross-sectional study carried out at the Center of Immunology and Biological Products in Camagüey, between January and December 2017. We evaluated 35 samples of umbilical cord blood obtained from newborns who were eutocic deliveries and whose mothers did not have infectious disease processes.

Results:

The highest average of mononuclear cells corresponded to lymphocytes. In the differential count, neutrophil polymorphonuclear cells ranked first, followed by lymphocytes, with averages of 0.50x109 and 0.46x109 per liter, respectively. Of the cells present in the button cell smear, lymphocytes were more frequent, with 0.59x109 per L; an average of monocytes was observed, with 0.00-0.07x109 per L was observed.

Conclusions:

Obtaining viable mononuclear cells through the umbilical vein is a promising technique in biomedical research. Among the mononuclear cells, lymphocytes predominated, both in the cord blood and in the isolated cell button.

Palavras-chave : umbilical cord; mononuclear cells; regenerative therapy.

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