Resumo

FERNANDEZ ROMERO, Tammy; GONZALEZ GRIEGO, Antonio; MELCHOR RODRIGUEZ, Antonio  e  FERNANDEZ ACOSTA, Félix J.. Obtention of a peroxidase-hepatitis B virus surface antigen conjugate . Rev Cubana Invest Bioméd [online]. 2007, vol.26, n.4, pp. 0-0. ISSN 1561-3011.

A conjugate was prepared for the determination of antibodies against the surface antigen of hepatitis B virus by double Ag sandwich ELISA. Peroxidase, the antigen of the Cuban vaccine against hepatitis B, and the periodate-conjugation method were used. The enzymatic activity, the immunological activity and the specific conjugate activity were evaluated. The enzyme and the antigen conserved their activity after conjugation, and the optimal working dilution 1/200 was selected. It was concluded that the conjugate obtained had an adequate specificity, detectability and reactivity, and could be considered apt for its use.

Palavras-chave : ELISA; conjugate evaluation; enzymatic activity; immunological activity; AgHBs; anti-HBs; hepatitis B.

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