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Pastos y Forrajes
versão impressa ISSN 0864-0394
Resumo
BECQUER, C.J et al. Selection of rhizobia adapted to livestock production ecosystems of Sancti Spiritus, Cuba, inoculated in corn (Zea mays L.).: Stage I: Greenhouse. Pastos y Forrajes [online]. 2008, vol.31, n.3, pp. 1-1. ISSN 0864-0394.
A greenhouse trial was carried out with the objective of selecting rhizobia strains inoculated in corn (Zea mays, L.). Thirty Cuban native strains belonging to Bradyrhizobium sp. were used, as well as 12 commercial strains belonging to different rhizobium genera and species. The inoculi were made in BYM medium and were shaken until reaching a titer of 106-108 cel./mL. The standard methods for cereal inoculation were used. The variables evaluated were: aerial dry weight (ADW), root dry weight (RDW), stem length (SL), stem diameter (SD) and presence of ears (PE). In the variables ADW, SL and SD treatments higher than the absolute control were found, and in the case of SL, two treatments showed values without significant differences with the fertilized control. No statistical differences were found among treatments in PE. In RDW the inoculated treatments were lower than the fertilized control, except JI2, TD1, SP7 and SP12. A grouping in clusters was obtained, by means of which the strains TD, JK, SP, SP, TE, JK, SP, USDA 110, JI, JJ, USDA 76, SP20, 25B6, NITRAGIN, ATCC 10317, were selected, which stood out to be evaluated later under field conditions. The treatments selected in the dendrogram constituted 69% for the native strains and 31% for the commercial ones. When evaluating the inoculated treatments regarding the degree of dependence of the different variables with regards to RDW, the regression index was determined to be weak, but when analyzing them separately, a strong regression was observed in the variables SD (native strains) and ADW (commercial strains). To continue these studies under field conditions is recommended.
Palavras-chave : Bradyrhizobium; inoculation; Zea mays.