Resumo

PETEIRA, Belkis; ESTEVES, Ivania; MONTES DE OCA, Nivian  e  HIDALGO-DIAZ, L. STABILITY OF IMI SD 187 Pochonia chlamydosporia var. catenulata STRAIN ON SOLID MEDIUM. Rev. Protección Veg. [online]. 2007, vol.22, n.2, pp. 124-127. ISSN 2224-4697.

A safe conservation method not affecting the strain characteristics is essential for developing any microorganism as a potential biological agent. The serial subculture is one of the most used methods. The aim of this work was to find out the effect of this practice on protease production by the strain IMI SD 187 of the nematophagous fungus Pochonia chlamydosporia var. catenulata. The study was carried out on solid medium by measuring the gelatine degradation halo, the fungal growth and the PA indicator (protease activity) at different days after inoculation. Adverse effects of serial subculture on protease production by the strain studied were not observed confirming the robustness of this strain.

Palavras-chave : proteases; solid medium; Pochonia chlamydosporia.

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