Resumo

GARCIA, M et al. ENTOMOPATHOGENIC NEMATODE PRODUCTION AT THE NATIONAL PHYTOSANITARY REFERENCE CENTER FOR THE MOUNTAIN IN CUBA. Rev. Protección Veg. [online]. 2007, vol.22, n.2, pp. 131-133. ISSN 2224-4697.

The entomopathogenic nematodes belonging to Heterorhabditis and Steinernema genera are efficient biological control agents for insects. Several strains of Heterorhabditis spp. have been studied and developed in Cuba, and they have been used successfully in pest management. In 2005, specialists from the National Center for Animal and Plant Health (CENSA) transferred the technology for mass rearing of entomopathogenic nematodes on Galleria mellonella to the National Phytosanitary Reference Center for the Mountain (CNRFM), and the native strain HC1 of Heterorhabditis bacteriophora was used to be evaluated in coffee berry borer (Hypotenemus hampei Ferrari) management. Seven batches of entomopathogenic nematodes were produced in the first three months and mean yields of 190 000 IJ larvae^-1 were obtained since batch No. 4, a value very close to the real productive potential of the strain. In relation to the viability (% of live juveniles), it was close to 90% since batch No. 2. As an indicator of a successful technology transfer, the specialists from CNRFM produced more than 291 millions of infestive juveniles (lJ) of the strain HC1 of H. bacteriophora. The lJ produced showed to be pathogenic to larvae of Spodoptera frugiperda (J.E. Smith), Diaphania hyalinata (L.) and Manduca sexta (L.) with 100% mortality at 48 hours.

Palavras-chave : Heterorhabditis bacteriophora; Hypotenemus hampei; coffee.

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