Resumo

CUELLO, Maribel et al. Validation of the ELISA for quantifying capsular anti-polysaccharide IgG of Neisseria meningitidis of the serogroup C in mice sera. Vaccimonitor [online]. 2001, vol.10, n.4, pp. 7-13. ISSN 1025-0298.

An indirect enzyme-linked immunoabsorbent assay (ELISA) was developed to determine antibody anti-polysaccharide C mouse IgG. Polystyrene plates precoating with poly-L-lysine, then Neisseria meningitidis group C polysaccharide (Finlay Institute, Havana, Cuba) was used to evaluated the immune response against this component in a candidate vaccine in pre-clinical studies. Alkaline phosphatase conjugate was used for recognizing mouse antibodies against polysaccharide C. The enzymatic reaction is evidenced by using the specific substrate p-nitrophenylphosphate. The detection limit of the assay was 123,74 U/mL, the specificity of assay was high. Intra-and interassay lack of precision, parallelism, linearity, and recovery were ± 10% of the expected values.

Palavras-chave : ELISA; Antipolysaccharide C; Neisseria meningitidis serogroup C; validation.

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