Resumo

ARENCIBIA, Daniel Francisco et al. Biomodels for micronucleis induction on bone marrow cells by cyclophosphamide and bleomycin. Vaccimonitor [online]. 2011, vol.20, n.1, pp. 28-33. ISSN 1025-0298.

Balb/c mice of both sexes were evaluated as biomodel in the induction of micronucleis in bone marrow cells by cyclophosphamide and bleomycin. They were divided into five experimental groups per sex. The first one was administered with NaCl 0.9% by intraperitoneal (i.p) route, the second and third groups were administered with cyclophosphamide by i.p route, with designs of different treatments at doses of 50 mg/kg. The fourth and fifth groups were administered with bleomycin by i.p route, equally in two designs of different treatments at 20 mg/kg doses. This resulted in a higher micronucleis induction of polychromatic erythrocytes and in a higher citotoxicity index with the use of cyclophosphamide administered twice before the sacrifice, with a 24-hours interval between administrations. According to our experimental conditions, this is the best design to induce a considerable number of micronucleis in bone marrow cells of mice, being useful in experimental designs to evaluate drugs with antigenotoxic effect. In addition, it implies its use according to the best found experimental design as positive control in mutagenesis and genotoxicity studies.

Palavras-chave : Induction; micronucleis; Balb-C mice; cyclophosphamide; bleomycin.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )