SciELO - Scientific Electronic Library Online

 
vol.21 número3Evaluación de la toxicidad por dosis repetida de un cocleato derivado de un proteoliposoma de Neisseria meningitidis en ratas Sprague DawleyPrescripción y vigilancia de la inmunoterapia sublingual con vacunas estandarizadas de ácaros domésticos en un servicio de alergología índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Vaccimonitor

versão impressa ISSN 1025-028X

Resumo

ROSARIO, Luis Alfredo et al. Phenotypic and molecular characterization of Leptospira clinical isolations from the Republic of Nicaragua. Vaccimonitor [online]. 2012, vol.21, n.3, pp. 6-12. ISSN 1025-028X.

This paper present the phenotypic and molecular characterization of eight strains of Leptospira isolated from Nicaragua clinical cases. The phenotypic characterization results showed absence of cell growth on culture media incubated at 13 °C or 8-azaguanina (2.25 mg/mL) supplemented medium. Besides a conversion to spherical shapes at 60 min to exposure NaCl 1M supplemented medium was observed. The molecular characterization was carried out by polymerase chain reaction (PCR) of ompL1 and lipL32 genes stored with pathogen strains. The results demonstrated the presence of amplified bands for both genes on eight strains, which allow to affirm that the analyzed microorganisms are Leptospira pathogens strains.

Palavras-chave : Leptospira ; phenotypic methods; PCR.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )